| 4 | 0 | 48 |
| 下载次数 | 被引频次 | 阅读次数 |
目的 探讨桥本甲状腺炎（HT）患者外周血微小核糖核酸-27a(mi R-27a)、白细胞介素-27(IL-27)水平及临床意义。方法 选取2022年1月至2024年2月收治的120例HT患者作为HT组，另选同期体检的健康者100例作为对照组。收集两组一般资料及甲状腺功能参数，检测并比较两组外周血mi R-27a、IL-27。采用Pearson相关分析mi R-27a、IL-27与甲状腺功能参数的关系，采用受试者工作特征（ROC）曲线分析mi R-27a、IL-27水平对HT的诊断价值。结果 HT组甲状腺疾病家族史占比、促甲状腺激素（TSH）、甲状腺球蛋白抗体（Tg Ab）、甲状腺过氧化物酶抗体（TPOAb）、mi R-27a、IL-27明显高于对照组，游离三碘甲状腺原氨酸（FT3）、游离甲状腺素（FT4）低于对照组（P<0.05,P<0.01）。miR-27a、IL-27水平与HT患者TSH、TgAb、TPOAb呈正相关（P<0.05,P<0.01），与HT患者FT3、FT4无明显相关性（P>0.05）。多因素logistic回归分析显示，有甲状腺疾病家族史、高水平Tg Ab、高水平TPOAb、高水平mi R-27a、高水平IL-27是导致HT发生的危险因素（P<0.05,P<0.01）。ROC曲线分析显示，mi R-27a、IL-27联合检测诊断HT的曲线下面积显著高于单独诊断（P<0.01）。结论 HT患者外周血mi R-27a、IL-27水平升高，且与患者甲状腺功能和抗体有关，mi R-27a、IL-27水平升高增加HT发生风险，对HT的早期诊断具有一定价值。
Abstract：Objective To investigate the expression and clinical significance of microrNA-27a(miR-27a) and interleukin-27(IL-27) in peripheral blood of patients with Hashimoto's thyroiditis(HT). Methods A total of 120 patients with HT admitted from January 2022 to February 2024 were selected as the HT group, and 100 healthy people who underwent physical examination during the same period were selected as the control group. The general data and thyroid function parameters of the two groups were collected, and miR-27a and IL-27 in peripheral blood were detected and compared between the two groups. Pearson correlation analysis was used to analyze the relationship between miR-27a, IL-27 and thyroid function parameters. Receiver operating characteristic(ROC) curve was used to analyze the diagnostic value of miR-27a and IL-27 levels in HT. Results The proportion of family history of thyroid disease, thyroid stimulating hormone(TSH), thyroglobulin antibody(TgAb), thyroid peroxidase antibody(TPOAb), miR-27a and IL-27 in the HT group were significantly higher than those in the control group.Free triiodothyronine(FT3) and free thyroxine(FT4) were lower than those in the control group(P<0.05 or P<0.01). The levels of miR-27a and IL-27 were positively correlated with TSH, TgAb and TPOAb in patients with HT(P<0.05 or P<0.01), but were not significantly correlated with FT3 and FT4 in patients with HT(P>0.05). Multivariate logistic regression analysis showed that family history of thyroid disease, high level of TgAb, high level of TPOAb, high level of miR-27a and high level of IL-27 were risk factors for HT(P<0.05 or P<0.01). ROC curve analysis showed that the area under the ROC curve of combined detection of miR-27a and IL-27 was significantly higher than that of single diagnosis(P<0.01). Conclusions The levels of miR-27a and IL-27in peripheral blood of patients with HT are highly expressed, and are related to thyroid function and antibodies. The elevated levels of miR-27a and IL-27 increase the risk of HT, and have certain value in the early diagnosis of HT.
[1] HU Y, FENG W, CHEN H, et al. Effect of selenium on thyroid autoimmunity and regulatory T cells in patients with Hashimoto's thyroiditis:a prospective randomized-controlled trial[J].Clin Transl Sci, 2021,14(4):1390-1402.
[2]韩静,刘守尧.桥本氏甲状腺炎中西医治疗研究进展[J].中华中医药杂志,2019,34(11):5327-5330.
[3] CAYRES L C F, DE SALIS L V V, RODRIGUES G S P,et al. Detection of alterations in the gut microbiota and intestinal permeability in patients with hashimoto thyroiditis[J].Front Immunol, 2021,12:579140.
[4] HANEGE F M, TUYSUZ O, CELIK S, et al. Hashimoto's thyroiditis in papillary thyroid carcinoma:a 22-year study[J].Acta Otorhinolaryngol Ital, 2021,41(2):142-145.
[5] KRYSIAK R, SZKRóBKA W, OKOPIE?B. The effect of gluten-free diet on thyroid autoimmunity in drug-na?ve women with hashimoto's thyroiditis:a pilot study[J].Exp Clin Endocrinol Diabetes, 2019,127(7):417-422.
[6] GAO Y, HAN D, FENG J. MicroRNA in multiple sclerosis[J].Clin Chim Acta, 2021,516:92-99.
[7] WU X, GU Q, CHEN X, et al. MiR-27a targets DKK2 and SFRP1to promote reosseointegration in the regenerative treatment of peri-implantitis[J].J Bone Miner Res, 2019,34(1):123-134.
[8] MACALINAO M L, INOUE S I, TSOGTSAIKHAN S,et al. IL-27 produced during acute malaria infection regulates Plasmodium-specific memory CD4+T cells[J].EMBO Mol Med,2023,15(12):e17713.
[9]滑雅娜,朱凌妍,白力,等.IL-27在RA中的作用机制分析[J].中国免疫学杂志,2021,37(9):1025-1028.
[10]中华医学会内分泌学分会《中国甲状腺疾病诊治指南》编写.中国甲状腺疾病诊治指南:甲状腺炎[J].中华内科杂志,2008,47(9):784-788.
[11] RUGGERI R M, GIOVINAZZO S, BARBALACE M C,et al. Influence of dietary habits on oxidative stress markers in Hashimoto's thyroiditis[J].Thyroid, 2021,31(1):96-105.
[12] HANNA H W Z, RIZZO C, ABDEL HALIM R M, et al. Vitamin D status in Hashimoto's thyroiditis and its association with vitamin D receptor genetic variants[J].J Steroid Biochem Mol Biol, 2021,212:105922.
[13]王晓娟,袁宁,孙娜,等.NLR、PLR联合检测在桥本甲状腺炎诊断中的临床价值分析[J].国际检验医学杂志,2022,43(14):1729-1732.
[14] MARTINO G, CAPUTO A, VICARIO C M, et al. Alexithymia,emotional distress, and perceived quality of life in patients with Hashimoto's thyroiditis[J].Front Psychol, 2021,12:667237.
[15]武明东,都广礼.补中益气汤加减治疗桥本甲状腺炎患者的临床研究[J].中成药,2020,42(12):3359-3361.
[16]李海燕,刘强,蒋丽,等.桥本甲状腺炎患者血清TpoAb、TgAb水平在诊断和分型中的应用价值[J].中国实验诊断学,2022,26(2):176-179.
[17] SEMPERE L F, AZMI A S, MOORE A. microRNA-based diagnostic and therapeutic applications in cancer medicine[J].Wiley Interdiscip Rev RNA, 2021,12(6):e1662.
[18]于龙.卡格列净对2型糖尿病肾病患者尿液外泌体miRNA、IL-1β和E-cadherin水平的影响[J].保健医学研究与实践,2022,19(4):13-15,31.
[19] YOU J, LI J, KE C, et al. Oncogenic long intervening noncoding RNA Linc00284 promotes c-Met expression by sponging miR-27a in colorectal cancer[J].Oncogene, 2021,40(24):4151-4166.
[20] FENG L, SHEN F, ZHOU J, et al. Hypoxia-induced up-regulation of miR-27a promotes paclitaxel resistance in ovarian cancer[J].Biosci Rep, 2020,40(4):BSR20192457.
[21] LIU J, LI X, LU S, et al. Glucagon-like peptide-1(GLP-1)improved diabetic lung fibrosis via AMPK and microRNA-27a(miR-27a)[J].Ann Transl Med, 2021,9(6):492.
[22] CHEN H, DONG Z, CHEN Y, et al. MicroRNA-27a promotes tumorigenesis in tongue squamous cell carcinoma by enhancing proliferation, migration and suppressing apoptosis[J].Eur Arch Otorhinolaryngol, 2021,278(11):4557-4567.
[23] LI Q, WANG B, MU K, et al. The pathogenesis of thyroid autoimmune diseases:new T lymphocytes:cytokines circuits beyond the Th1-Th2 paradigm[J].J Cell Physiol, 2019,234(3):2204-2216.
[24] HEPP M, WERION A, DE GREEF A, et al. Oxidative stressinduced sirtuin1 downregulation correlates to HIF-1α, GLUT-1,and VEGF-A Upregulation in Th1 autoimmune Hashimoto's thyroiditis[J].Int J Mol Sci, 2021,22(8):3806.
[25] HU M, WEI J, YANG L, et al. Linc-KIAA1737-2 promoted LPSinduced HK-2 cell apoptosis by regulating miR-27a-3p/TLR4/NF-κB axis[J].J Bioenerg Biomembr, 2021,53(4):393-403.
[26] CAI C, MIN S, YAN B, et al. MiR-27a promotes the autophagy and apoptosis of IL-1β treated-articular chondrocytes in osteoarthritis through PI3K/AKT/mTOR signaling[J].Aging(Albany NY), 2019,11(16):6371-6384.
[27] ZHU L, FENG Z, ZHANG J, et al. MicroRNA-27a regulates ferroptosis through SLC7A11 to aggravate cerebral ischemiareperfusion injury[J].Neurochem Res, 2023,48(5):1370-1381.
基本信息:
DOI：
中图分类号:R581.4
引用信息:
[1]张志平,房艳,刘家云.桥本甲状腺炎患者外周血miR-27a、IL-27水平及临床意义[J].临床误诊误治,2025,38(11):19-23.
基金信息:
国家重点研发计划课题项目（2022YFC3602302）